Slide method for detection of antibody-coated bacteria in urine sediments

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Slide method for detection of antibody-coated bacteria in urine sediments.

An immunofluorescent slide method incorporating 0.1% (wt/vol) Evans blue as a counterstain was developed and compared with a recently described tube method. Seventy-one urine specimens were tested concurrently by both methods. Results of the two methods agreed in 70 specimens and disagreed in only 1. We found the slide method to be less costly and time consuming than the tube method.

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Further evaluation of the test for detection of antibody-coated bacteria in urine sediment.

To assess the reproducibility of the test for detection of antibody-coated bacteria in urine sediment as it might be used in a diagnostic laboratory for classification of urinary tract infections, multiple urine specimens from 83 patients were tested. The results were reproducible and consistent, or if inconsistent potentially explainable, in all but four patients. The explanation for inconsist...

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Antibody coated bacteria in urine of patients with recent spinal injury.

Twenty patients with an acute spinal injury were prospectively studied to assess the clinical importance of antibody coated bacteria (ACB) in the urine and the association among the different bacterial species with a positive antibody coated bacteria test. Clinical urinary tract infection was associated with a positive ACB test on 45% of occasions. Three hundred and ninety nine urine samples co...

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Diagnostic value and biological significance of antibody-coated bacteria in urine.

The incidence of antibody-coated bacteria (ACB) in the urinary sediments as an indication of the site of urinary tract infections (UTI) was investigated in 103 adult subjects with persistent bacteriuria by means of a direct immunofluorescence technique.ACB were found in 49 of 58 (84.5%) subjects with long-standing upper urinary tract obstruction and in 5 of 45 (11.1%) with lower UTI; this diffe...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1980

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.12.6.761-763.1980